Details
- Autor(en) / Beteiligte
- Titel
- Next-generation GRAB sensors for monitoring dopaminergic activity in vivo
- Ist Teil von
- Nature methods, 2020-11, Vol.17 (11), p.1156
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dopamine (DA) plays a critical role in the brain, and the ability to directly measure dopaminergic activity is essential for understanding its physiological functions. We therefore developed red fluorescent G-protein-coupled receptor-activation-based DA (GRAB ) sensors and optimized versions of green fluorescent GRAB sensors. In response to extracellular DA, both the red and green GRAB sensors exhibit a large increase in fluorescence, with subcellular resolution, subsecond kinetics and nanomolar-to-submicromolar affinity. Moreover, the GRAB sensors resolve evoked DA release in mouse brain slices, detect evoked compartmental DA release from a single neuron in live flies and report optogenetically elicited nigrostriatal DA release as well as mesoaccumbens dopaminergic activity during sexual behavior in freely behaving mice. Coexpressing red GRAB with either green GRAB or the calcium indicator GCaMP6s allows tracking of dopaminergic signaling and neuronal activity in distinct circuits in vivo.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1548-7105DOI: 10.1038/s41592-020-00981-9Titel-ID: cdi_pubmed_primary_33087905
- Format
- –
- Schlagworte
- Animals, Biosensing Techniques - methods, Brain - metabolism, Dopamine - metabolism, Drosophila - genetics, Drosophila - metabolism, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, HEK293 Cells, Humans, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, Mice, Neurons - metabolism, Rats, Receptors, Dopamine - genetics, Receptors, Dopamine - metabolism, Receptors, G-Protein-Coupled - genetics, Receptors, G-Protein-Coupled - metabolism, Red Fluorescent Protein, Sexual Behavior - physiology