Details

Autor(en) / Beteiligte

• Hachim, Asmaa
• Kavian, Niloufar
• Cohen, Carolyn A
• Chin, Alex W H
• Chu, Daniel K W
• Mok, Chris K P
• Tsang, Owen T Y
• Yeung, Yiu Cheong
• Perera, Ranawaka A P M
• Poon, Leo L M
• Peiris, J S Malik
• Valkenburg, Sophie A

Titel

ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection

Ist Teil von

• Nature immunology, 2020-10, Vol.21 (10), p.1293-1301

Ort / Verlag

United States

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.