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Assessing Immune Responses in the Nonobese Diabetic Mouse Model of Type 1 Diabetes
Ist Teil von
Methods in molecular biology (Clifton, N.J.), 2020, Vol.2128, p.269
Ort / Verlag
United States
Erscheinungsjahr
2020
Quelle
MEDLINE
Beschreibungen/Notizen
Type 1 diabetes is an autoimmune disease resulting in the loss of insulin production and, consequently, hyperglycemia. The nonobese diabetic (NOD) mouse develops spontaneous diabetes with considerable similarity to the disease in humans. Immunological studies using the NOD mouse model allow for the investigation of the natural history of the disease and leukocyte and lymphocyte pathogenic and regulatory functions, as well as testing potential therapies for intervention. The analyses of the cellular events leading up to diabetes may utilize different in vitro cellular assays, immunohistochemistry, and in vivo adoptive transfer, to study mechanisms of the disease and the effects of therapeutic intervention. In this chapter, we describe some common techniques for phenotyping and mechanistic analyses of function, particularly of CD8+ T cells.