Details
- Autor(en) / Beteiligte
- Titel
- Isolation and identification of Arbovirus in Hainan province, 2017-2018
- Ist Teil von
- Zhōnghuá liúxíngbìng zázhì, 2020-02, Vol.41 (2), p.236
- Ort / Verlag
- China
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To understand the types and distribution of Arboviruses in Hainan province. Blood-sucking insects were collected in Hainan province from 2017 to 2018. After laboratory treatment, BHK-21 cells and C6/36 cells were inoculated with grinding supernatant of all blood-sucking insects to isolate all of involving virus. Arbovirus genes in blood-sucking insects were detected in parallel by RT-PCR method. A total of 15 062 mosquitoes were classified into four genera (Culex, Armigeres, Aedes, Anopheles) and 11 360 midges were collected. was in the majority and accounted for 92.88 (13 990/15 062) of all the mosquitoes collected. Four strains of virus isolates were notified by tissue culture method. Three strains of viruses belonged to Japanese encephalitis virus (JEV), with the other one as Getah virus (GETV). Five pools of JEV gene amplification were positive, from Culex tritaeniorhynchus. Results from the phylogenetic analysis showed that they belonged to genotype JEV-Ⅰ. The minimum infection rate of JEV was 0.57‰ (8/1
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0254-6450DOI: 10.3760/cma.j.issn.0254-6450.2020.02.018Titel-ID: cdi_pubmed_primary_32164136
- Format
- –
- Schlagworte
- Alphavirus - genetics, Alphavirus - isolation & purification, Animals, Arboviruses - genetics, Arboviruses - isolation & purification, China, Culex - virology, Culicidae - virology, Encephalitis Virus, Japanese - genetics, Encephalitis Virus, Japanese - isolation & purification, Humans, Phylogeny