Details

Autor(en) / Beteiligte

• Field, Natania S
• O'Leary, Claire E
• Dybas, Joseph M
• Ding, Hua
• Oliver, Paula M

Titel

An Integrated Strategy for Identifying Targets of Ubiquitin-Mediated Degradation in CD4+ T Cells

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2020-01, Vol.2111, p.239

Ort / Verlag

United States

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Ubiquitination is a crucial component of many immune processes. While ubiquitin-mediated degradation is essential to T cell activation via T cell receptor signaling, the specific E3 ligases and substrates involved are not well-understood. Here, we describe a strategy integrating RNA, protein, and posttranslational modification datasets to identify targets of ubiquitin-mediated degradation. When integrated, these assays can provide broad insight into how this posttranslational modification regulates protein function and influences T cell biology.