Details

Autor(en) / Beteiligte

• Ding, Ning
• Zhang, Xin
• Zhang, Xue Di
• Jing, Jun
• Liu, Shan Shan
• Mu, Yun Ping
• Peng, Li Li
• Yan, Yun Jing
• Xiao, Geng Miao
• Bi, Xin Yun
• Chen, Hao
• Li, Fang Hong
• Yao, Bing
• Zhao, Allan Z

Titel

Impairment of spermatogenesis and sperm motility by the high-fat diet-induced dysbiosis of gut microbes

Ist Teil von

• Gut, 2020-09, Vol.69 (9), p.1608-1619

Ort / Verlag

England: BMJ Publishing Group LTD

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• ObjectiveHigh-fat diet (HFD)-induced metabolic disorders can lead to impaired sperm production. We aim to investigate if HFD-induced gut microbiota dysbiosis can functionally influence spermatogenesis and sperm motility.DesignFaecal microbes derived from the HFD-fed or normal diet (ND)-fed male mice were transplanted to the mice maintained on ND. The gut microbes, sperm count and motility were analysed. Human faecal/semen/blood samples were collected to assess microbiota, sperm quality and endotoxin.ResultsTransplantation of the HFD gut microbes into the ND-maintained (HFD-FMT) mice resulted in a significant decrease in spermatogenesis and sperm motility, whereas similar transplantation with the microbes from the ND-fed mice failed to do so. Analysis of the microbiota showed a profound increase in genus Bacteroides and Prevotella, both of which likely contributed to the metabolic endotoxaemia in the HFD-FMT mice. Interestingly, the gut microbes from clinical subjects revealed a strong negative correlation between the abundance of Bacteroides-Prevotella and sperm motility, and a positive correlation between blood endotoxin and Bacteroides abundance. Transplantation with HFD microbes also led to intestinal infiltration of T cells and macrophages as well as a significant increase of pro-inflammatory cytokines in the epididymis, suggesting that epididymal inflammation have likely contributed to the impairment of sperm motility. RNA-sequencing revealed significant reduction in the expression of those genes involved in gamete meiosis and testicular mitochondrial functions in the HFD-FMT mice.ConclusionWe revealed an intimate linkage between HFD-induced microbiota dysbiosis and defect in spermatogenesis with elevated endotoxin, dysregulation of testicular gene expression and localised epididymal inflammation as the potential causes.Trial registration number NCT03634644.