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The objectives of study were to explore the effects of exogenous methyltestosterone( MT) on the growth and gonadal development of overwintering Whitmania pigra. Before overwintering,0. 1,1. 0,10. 0,100. 0,150. 0 μg·L
of MT were added to the aquaculture water for 6 weeks. The changes of growth performance,gonad index,endogenous steroid hormones level and internal quality were measured after hibernate for 60 days. Then the tissue slice technique was used to observe the spermary( ovary) of Wh. pigra.The results showed that the body weight,survival rate and gonadal index increased first and then decreased with the increase of exogenous MT concentration; the male gland index was found the highest at the concentration of MT 10. 0 μg·L
and the female gland index was the highest at the concentration of MT 1. 0 μg·L
. The survival rate of Wh. pigra peaked at the concentration of MT 10. 0 μg·L
.The weight reaches a peak at a concentration of MT 100. 0 μg·L
( P<0. 05). The number of primary spermatocytes in the testis w