Zhongguo gu shang, 2019-11, Vol.32 (11), p.1026
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Effects of macrophage with Wnt5a over-expression on inflammation and chondrogenic differentiation in co-culture system
- Ist Teil von
- Zhongguo gu shang, 2019-11, Vol.32 (11), p.1026
- Ort / Verlag
- China
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To construct and compare macrophage by gene modification of Wnt5a which co-cultured by human bone marrow-derived mesenchymal stem cells (MSCs) with two dimension (2D) and bone marrow aspirates in vitr transwell, in order to investigate the effect of Wnt5a signaling on cartilage homeostasis through regulation of macrophage pro-inflammatory responses. Macrophages, MSCs and bone marrow aspirates specimens were extracted from 6 patients with severe knee deformities undergoing total knee arthroplasty(2 males, 4 females, aged from 58 to 71 years old) from September 2015 to December 2018. The synovial tissues of knee joints were exposed to type II collagenase and obtained single cell suspensions, and the purity of macrophages was determined by Ficoll gradient centrifugation and anti-CD14 antibody flow cytometry. The macrophages were transduced with IFN-γ combined with TNF-α for 48 h, and rAAV-lacZ or Wnt5a transfected for 24 h, then co-cultured model by human bone marrow-derived mesenchymal stem cells (MSCs) with tw
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1003-0034DOI: 10.3969/j.issn.1003-0034.2019.11.010Titel-ID: cdi_pubmed_primary_31870051
- Format
- –
- Schlagworte
- Aged, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Chondrocytes, Chondrogenesis, Coculture Techniques, Female, Humans, Inflammation, Macrophages, Male, Middle Aged, Wnt-5a Protein