Details

Autor(en) / Beteiligte

• Santos, Evelyn C. S
• dos Santos, Thiago C
• Fernandes, Tamires S
• Jorge, Fernanda L
• Nascimento, Vanessa
• Madriaga, Vinicius G. C
• Cordeiro, Pâmella S
• Checca, Noemi R
• Da Costa, Nathalia Meireles
• Pinto, Luís Felipe Ribeiro
• Ronconi, Célia M

Titel

A reversible, switchable pH-driven quaternary ammonium pillar[5]arene nanogate for mesoporous silica nanoparticles

Ist Teil von

• Journal of materials chemistry. B, Materials for biology and medicine, 2020-01, Vol.8 (4), p.73-714

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• Here we describe the assembly and pH-driven operation of two nanocarriers based on non-functionalized (MCM-41) and carboxylate-functionalized (MCM-41-COOH) containers loaded with the anticancer drug doxorubicin (DOX) and capped by quaternary ammonium pillar[5]arene (P[5]A) nanogates. MCM-41 and MCM-41-COOH containers were synthesized and transmission and scanning electron microscopies showed nanoparticles with spherical morphology and dimensions of 85 ± 13 nm. The nanochannels of MCM-41 loaded with DOX were gated through the electrostatic interactions between P[5]A and the silanolate groups formed at the silica-water interface, yielding the MCM-41-DOX-P[5]A nanocarrier. The second nanocarrier was gated through the electrostatic interactions between the carboxylate groups mounted on the surface of MCM-41 and P[5]A, resulting in the MCM-41-COO-DOX-P[5]A nanocarrier. The DOX release profiles from both nanocarriers were investigated by UV-vis spectroscopy at different pH values (2.0, 5.5 and 7.4) and also in the presence of ions, such as citrate 3− (19 mmol L −1 ) and Zn 2+ (1.2 and 50 mmol L −1 ) at 37 °C. MCM-41-COO-DOX-P[5]A can be turned on and off eight times through the formation and breaking of electrostatic interactions. In vitro studies show that MCM-41-COO-DOX-P[5]A can penetrate and release DOX in the nucleus of human breast adenocarcinoma MCF-7 cancer cells leading to a pronounced cytotoxic effect. Therefore, the fabricated nanocarrier based on a water-soluble cationic pillar[5]arene nanogate, which is reversibly opened and closed by electrostatic interactions, can be considered as a promising drug transport and delivery technique for future cancer therapy. Two nanoreservoirs based on non-functionalized (MCM-41) and carboxylate-functionalized (MCM-41-COO − ) loaded with the anticancer drug doxorubicin (DOX) and capped by quaternary ammonium pillar[5]arene (P[5]A) nanogates were constructed.