Details

Autor(en) / Beteiligte

• Kwon, Paul S
• Ren, Shaokang
• Kwon, Seok-Joon
• Kizer, Megan E
• Kuo, Lili
• Xie, Mo
• Zhu, Dan
• Zhou, Feng
• Zhang, Fuming
• Kim, Domyoung
• Fraser, Keith
• Kramer, Laura D
• Seeman, Nadrian C
• Dordick, Jonathan S
• Linhardt, Robert J
• Chao, Jie
• Wang, Xing

Titel

Designer DNA architecture offers precise and multivalent spatial pattern-recognition for viral sensing and inhibition

Ist Teil von

• Nature chemistry, 2020-01, Vol.12 (1), p.26-35

Ort / Verlag

England

Erscheinungsjahr

2020

Quelle

MEDLINE

Beschreibungen/Notizen

• DNA, when folded into nanostructures with a specific shape, is capable of spacing and arranging binding sites into a complex geometric pattern with nanometre precision. Here we demonstrate a designer DNA nanostructure that can act as a template to display multiple binding motifs with precise spatial pattern-recognition properties, and that this approach can confer exceptional sensing and potent viral inhibitory capabilities. A star-shaped DNA architecture, carrying five molecular beacon-like motifs, was constructed to display ten dengue envelope protein domain III (ED3)-targeting aptamers into a two-dimensional pattern precisely matching the spatial arrangement of ED3 clusters on the dengue (DENV) viral surface. The resulting multivalent interactions provide high DENV-binding avidity. We show that this structure is a potent viral inhibitor and that it can act as a sensor by including a fluorescent output to report binding. Our molecular-platform design strategy could be adapted to detect and combat other disease-causing pathogens by generating the requisite ligand patterns on customized DNA nanoarchitectures.