Nanoscale, 2019-11, Vol.11 (43), p.2884-2892
2019
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- Autor(en) / Beteiligte
- Titel
- Aptamer-functionalized exosomes from bone marrow stromal cells target bone to promote bone regeneration
- Ist Teil von
- Nanoscale, 2019-11, Vol.11 (43), p.2884-2892
- Ort / Verlag
- England: Royal Society of Chemistry
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In elderly people particularly in postmenopausal women, inadequate bone formation by osteoblasts originating from bone marrow mesenchymal stem cells (BMSCs) for compensation of bone resorption by osteoclasts is a major reason for osteoporosis. Enhancing osteoblastic differentiation of BMSCs is a feasible therapeutic strategy for osteoporosis. Here, bone marrow stromal cell (ST)-derived exosomes (STExos) are found to remarkably enhance osteoblastic differentiation of BMSCs in vitro . However, intravenous injection of STExos is inefficient in ameliorating osteoporotic phenotypes in an ovariectomy (OVX)-induced postmenopausal osteoporosis mouse model, which may be because STExos are predominantly accumulated in the liver and lungs, but not in bone. Hereby, the STExo surface is conjugated with a BMSC-specific aptamer, which delivers STExos into BMSCs within bone marrow. Intravenous injection of the STExo-Aptamer complex enhances bone mass in OVX mice and accelerates bone healing in a femur fracture mouse model. These results demonstrate the efficiency of BMSC-specific aptamer-functionalized STExos in targeting bone to promote bone regeneration, providing a novel promising approach for the treatment of osteoporosis and fracture. A novel strategy to deliver therapeutic exosomes to bone is developed for the first time by conjugating a specific BMSC-targeting aptamer to the exosomal surface.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2040-3364eISSN: 2040-3372DOI: 10.1039/c9nr02791bTitel-ID: cdi_pubmed_primary_31660556
- Format
- –
- Schlagworte
- Animals, Aptamers, Nucleotide - chemistry, Aptamers, Nucleotide - metabolism, Biomedical materials, Bone Density, Bone marrow, Bone Marrow Cells - cytology, Bone Regeneration, Cell Differentiation, Differentiation, Disease Models, Animal, Exosomes - genetics, Exosomes - metabolism, Exosomes - transplantation, Femur, Femur - diagnostic imaging, Flow Cytometry, Fractures, Hormone replacement therapy, Male, Mesenchymal Stem Cells - cytology, Mesenchymal Stem Cells - metabolism, Mice, Mice, Inbred C57BL, Mice, Transgenic, MicroRNAs - metabolism, Older people, Osteoblasts, Osteoclasts - cytology, Osteoclasts - metabolism, Osteoporosis, Osteoporosis - therapy, Regeneration (physiology), Stem cells, Tissue Distribution, X-Ray Microtomography