Details

Autor(en) / Beteiligte

• Meyer, James O
• Dahimene, Shehrazade
• Page, Karen M
• Ferron, Laurent
• Kadurin, Ivan
• Ellaway, Joseph I J
• Zhao, Pengxiang
• Patel, Tarun
• Rothwell, Simon W
• Lin, Peipeng
• Pratt, Wendy S
• Dolphin, Annette C

Titel

Disruption of the Key Ca 2+ Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel Trafficking

Ist Teil von

• Cell reports (Cambridge), 2019-10, Vol.29 (1), p.22

Ort / Verlag

United States

Erscheinungsjahr

2019

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Voltage-gated calcium channels are exquisitely Ca selective, conferred primarily by four conserved pore-loop glutamate residues contributing to the selectivity filter. There has been little previous work directly measuring whether the trafficking of calcium channels requires their ability to bind Ca in the selectivity filter or to conduct Ca . Here, we examine trafficking of neuronal Ca 2.1 and 2.2 channels with mutations in their selectivity filter and find reduced trafficking to the cell surface in cell lines. Furthermore, in hippocampal neurons, there is reduced trafficking to the somatic plasma membrane, into neurites, and to presynaptic terminals. However, the Ca 2.2 selectivity filter mutants are still influenced by auxiliary α δ subunits and, albeit to a reduced extent, by β subunits, indicating the channels are not grossly misfolded. Our results indicate that Ca binding in the pore of Ca 2 channels may promote their correct trafficking, in combination with auxiliary subunits. Furthermore, physiological studies utilizing selectivity filter mutant Ca channels should be interpreted with caution.