Methods in molecular biology (Clifton, N.J.), 2020-01, Vol.2072, p.129
2020
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Details
- Autor(en) / Beteiligte
- Titel
- Genome-Wide Identification of Allele-Specific Gene Expression in a Parent-of-Origin Specific Manner
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2020-01, Vol.2072, p.129
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Upon fertilization, normal endosperm and embryo development require the contribution of both the maternal and paternal genomes. However, certain genes are expressed in a parent-of-origin-dependent manner, an epigenetic phenomenon known as genomic imprinting. Despite the blast of new technologies and the crucial advances of the past decades in the epigenetics field, novel imprinted genes are yet to be discovered and thus key regulators of early seed development. Using rice plant as a model, we describe a method for the identification of imprinted genes based on an RNA-Seq approach, which allows the identification of maternal and paternal gene expression in a parent-of-origin-specific manner.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-9865-4_11Titel-ID: cdi_pubmed_primary_31541443
- Format
- –
- Schlagworte
- Alleles, Crosses, Genetic, Endosperm - genetics, Gene Expression, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene Library, Genes, Plant, Genome, Plant, Genomics, Oryza - genetics, Plant Breeding, Plant Development, Polymorphism, Single Nucleotide, Seeds - genetics