Details

Autor(en) / Beteiligte

• Jung, Il Seon
• Jeon, Min Gyu
• Oh, Da Som
• Jung, Ye Jin
• Kim, Hyun-Su
• Bae, Donghyuck
• Kim, Yujin
• Lee, Gyeong-Eun
• Choi, Chulyung
• Hwang, Yong Pil

Titel

Micronized, Heat-Treated Lactobacillus plantarum LM1004 Alleviates Cyclophosphamide-Induced Immune Suppression

Ist Teil von

• Journal of medicinal food, 2019-09, Vol.22 (9), p.896-906

Ort / Verlag

United States

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• The present study investigated the immunomodulatory activity and associated mechanisms of heat-treated LM1004 (HT-LM1004) in a cyclophosphamide (CTX)-induced mouse model of immunosuppression. HT-LM1004 induced phagocytic activity and nitric oxide production in RAW264.7 macrophages and stimulated the release of tumor necrosis factor (TNF)- , interferon (IFN)- , interleukin (IL)-2, and IL-12p70. In mice with CTX-induced immunosuppression, oral HT-LM1004 administration restored thymus and spleen indices, including spleen weight. Consistent with the results, HT-LM1004 increased TNF- , IFN- , IL-2, and IL-12p70 levels in mice after 14 days of treatment and enhanced the natural killer (NK) cell activity of splenocytes from mice with CTX-induced immunosuppression against YAC-1 lymphoma cells. The method of HT-LM1004 generation influenced this activity: LM1004 grown in a membrane bioreactor, which reduced the size of the cells to <1.0 μm through physical stress (micronization), promoted NK cell cytotoxicity to a greater extent than LM1004 subjected to heat treatment alone. These findings indicate that HT-LM1004 without or with micronization can reverse CTX-induced immunosuppression without adverse side effects by potentiating NK cell function.