Details

Autor(en) / Beteiligte

• Liu, Jin-Fang
• Zhao, Ke
• Gao, Hui-Ying
• Gao, Rui
• Zhan, Yi-Qun
• Yang, Xiao-Ming
• Li, Chang-Yan
• Wei, Kai-Hua

Titel

Dynamics of Proliferation and Differentiation after Transplantation of Hematopoietic Cells in Mouse

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2019-06, Vol.27 (3), p.958

Ort / Verlag

China

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effects of Listeria monocytogenes infection on hematopoietic stem and progenitor cell (HSPC) composition, cell cycle and cell colony-forming ability in mouse bone marrow. The C57BL/6J mice were divided into infected group and control group. The mice in injected group were infected intraperitoneally with 6.7×10 CFU Listeria monocytogenes,while the mice in control group were injecfed with PBS of same volume.The serum levels of IFNγ were detected at different time points. After 24 hours, the HS/PC composition, cell cycle and cell colony-forming ability in bone marrow of mice were measured, and the difference between the control group and the infected group was statistically analyzed. Serum IFNγ levels peaked at 24 hours after infection with Listeria monocytogenes. After 24 h, the proportion of LSK, LSK in S phase, and short-term hematopoietic stem cells (ST-HSC) in the infected group were significantly higher than those in the control group (P＜0.001), long-term hematopoietic stem cells (LT-HSC