Nature methods, 2019-06, Vol.16 (6), p.533-544
2019
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Details
- Autor(en) / Beteiligte
- Titel
- SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues
- Ist Teil von
- Nature methods, 2019-06, Vol.16 (6), p.533-544
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Fluorescence in situ hybridization (FISH) reveals the abundance and positioning of nucleic acid sequences in fixed samples. Despite recent advances in multiplexed amplification of FISH signals, it remains challenging to achieve high levels of simultaneous amplification and sequential detection with high sampling efficiency and simple workflows. Here we introduce signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short complementary fluorescent imager strands. We show that SABER amplified RNA and DNA FISH signals (5- to 450-fold) in fixed cells and tissues. We also applied 17 orthogonal amplifiers against chromosomal targets simultaneously and detected mRNAs with high efficiency. We then used 10-plex SABER-FISH to identify in vivo introduced enhancers with cell-type-specific activity in the mouse retina. SABER represents a simple and versatile molecular toolkit for rapid and cost-effective multiplexed imaging of nucleic acid targets.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/s41592-019-0404-0Titel-ID: cdi_pubmed_primary_31110282
- Format
- –
- Schlagworte
- Animals, Cells, Cultured, DNA - analysis, DNA - genetics, DNA, Single-Stranded - chemistry, Fluorescent Dyes - metabolism, Humans, In Situ Hybridization, Fluorescence - methods, Mice, Oligonucleotides - chemistry, Optical Imaging - methods, Retina - diagnostic imaging, Retina - metabolism, RNA - analysis, RNA - genetics