Details

Autor(en) / Beteiligte

• Sun, Zhen
• Zhao, Jing
• Yu, Hua
• Zhang, Chenyang
• Li, Hu
• Zeng, Zhongda
• Zhang, Jin

Titel

Metabolomics in Stem Cell Biology Research

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2019, Vol.1975, p.321

Ort / Verlag

United States

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Stem cell research has been greatly facilitated by comprehensive and integrative multi-omics studies. As a unique approach of functional analysis, metabolomics measures many metabolites and activities of metabolic pathways which can directly indicate cellular energetic status, cell proliferation and fitness, and stem cell fate choices such as self-renewal versus differentiation. Here we describe the methods of applying metabolomics, C-labeled glucose and glutamine tracing with mouse embryonic stem cells (ES cells), metabolite analysis using mass spectrometry tools, and the following statistical and computational modeling analysis. Integration of these methods into the more common gene expression and epigenetics analysis toolbox will help to generate a more complete picture and in-depth understanding of one's stem cells of interest.