Details

Autor(en) / Beteiligte

• Kienitz, Marie-Cecile
• Niemeyer, Anne
• König, Gabriele M
• Kostenis, Evi
• Pott, Lutz
• Rinne, Andreas

Titel

Biased signaling of Ca 2+ -sensing receptors in cardiac myocytes regulates GIRK channel activity

Ist Teil von

• Journal of molecular and cellular cardiology, 2019-05, Vol.130, p.107

Ort / Verlag

England

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Ca -sensing receptors (CaSRs) belong to the class C of G protein-coupled receptors and are activated by extracellular Ca . CaSRs display biased G protein signaling by coupling to different classes of heterotrimeric G proteins depending on agonist and cell type. In this study we used fluorescent biosensors to directly analyze G protein coupling to CaSRs and downstream signaling in living cells. In HEK 293 cells, CaSRs displayed biased signaling: elevation of extracellular Ca or application of the alternative agonist spermine caused activation of G - and G -proteins. Adult cardiac myocytes express endogenous CaSRs, which have been implicated in regulating Ca signaling and contractility. Biased signaling of CaSRs has not been investigated in these cells. To evaluate efficiencies of G - and G -signaling via CaSRs in rat atrial myocytes, we measured G protein-activated K (GIRK) channels. Activation of GIRK requires binding of Gβγ subunits released from G proteins, whereas G -signaling results in inhibition of GIRK channel activity. Stimulation of CaSRs by Ca or spermine failed to directly activate G and GIRK channels. When GIRK channels were pre-activated via endogenous M receptors, stimulation of CaSRs caused pronounced inhibition of GIRK currents. This effect was specific to CaSR activation: GIRK current inhibition was sensitive to NPS-2143, a negative allosteric modulator of CaSRs, and abrogated by FR900359, a direct inhibitor of G . GIRK current inhibition was also sensitive to the PKC inhibitor chelerythrine, suggesting that following activation of CaSR and G , GIRK currents are modulated by PKC phosphorylation. We conclude from this data that cardiac CaSRs do not activate G and affect GIRK currents preferentially via the G /PKC pathway.