Details

Autor(en) / Beteiligte

• Li, Tiehai
• Liu, Lin
• Wei, Na
• Yang, Jeong-Yeh
• Chapla, Digantkumar G
• Moremen, Kelley W
• Boons, Geert-Jan

Titel

An automated platform for the enzyme-mediated assembly of complex oligosaccharides

Ist Teil von

• Nature chemistry, 2019-03, Vol.11 (3), p.229-236

Ort / Verlag

England

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• An automated platform that can synthesize a wide range of complex carbohydrates will greatly increase their accessibility and should facilitate progress in glycoscience. Here we report a fully automated process for enzyme-mediated oligosaccharide synthesis that can give easy access to different classes of complex glycans including poly-N-acetyllactosamine derivatives, human milk oligosaccharides, gangliosides and N-glycans. Our automated platform uses a catch and release approach in which glycosyltransferase-catalysed reactions are performed in solution and product purification is accomplished by solid phase extraction. We developed a sulfonate tag that can easily be installed and enables highly efficient solid phase extraction and product release using a single set of washing conditions, regardless of the complexity of the glycan. Using this custom-built synthesizer, as many as 15 reaction cycles can be performed in an automated fashion without a need for lyophilization or buffer exchange steps.