Details

Autor(en) / Beteiligte

• Ganor, Yonatan
• Real, Fernando
• Sennepin, Alexis
• Dutertre, Charles-Antoine
• Prevedel, Lisa
• Xu, Lin
• Tudor, Daniela
• Charmeteau, Bénédicte
• Couedel-Courteille, Anne
• Marion, Sabrina
• Zenak, Ali-Redha
• Jourdain, Jean-Pierre
• Zhou, Zhicheng
• Schmitt, Alain
• Capron, Claude
• Eugenin, Eliseo A
• Cheynier, Rémi
• Revol, Marc
• Cristofari, Sarra
• Hosmalin, Anne
• Bomsel, Morgane

Titel

HIV-1 reservoirs in urethral macrophages of patients under suppressive antiretroviral therapy

Ist Teil von

• Nature microbiology, 2019-04, Vol.4 (4), p.633-644

Ort / Verlag

England

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Human immunodeficiency virus type 1 (HIV-1) eradication is prevented by the establishment on infection of cellular HIV-1 reservoirs that are not fully characterized, especially in genital mucosal tissues (the main HIV-1 entry portal on sexual transmission). Here, we show, using penile tissues from HIV-1-infected individuals under suppressive combination antiretroviral therapy, that urethral macrophages contain integrated HIV-1 DNA, RNA, proteins and intact virions in virus-containing compartment-like structures, whereas viral components remain undetectable in urethral T cells. Moreover, urethral cells specifically release replication-competent infectious HIV-1 following reactivation with the macrophage activator lipopolysaccharide, while the T-cell activator phytohaemagglutinin is ineffective. HIV-1 urethral reservoirs localize preferentially in a subset of polarized macrophages that highly expresses the interleukin-1 receptor, CD206 and interleukin-4 receptor, but not CD163. To our knowledge, these results are the first evidence that human urethral tissue macrophages constitute a principal HIV-1 reservoir. Such findings are determinant for therapeutic strategies aimed at HIV-1 eradication.