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New-Generation Chemical Tools for the Manipulation of Auxin Biosynthesis, Action, and Transport
Ist Teil von
Methods in molecular biology (Clifton, N.J.), 2019, Vol.1924, p.143
Ort / Verlag
United States
Erscheinungsjahr
2019
Quelle
MEDLINE
Beschreibungen/Notizen
Auxin is the master regulator for almost every aspect of plant growth and development. Small molecule inhibitors, fluorescently labeled molecule, and hormone analogs on auxin biosynthesis, transport, and signaling, so-called auxin chemical tools, have been widely utilized to dissect physiological functions of gene families in auxin biosynthesis, transport, and signaling. Auxin chemical tools can manipulate specific auxin-regulated events at any developmental stage. Chemical tools can modulate the function of orthologs of target proteins and also can overcome the redundant function of large family gene controlling auxin-regulated response. On the other hand, chemical tool might induce the off-target effects at high concentration, if the chemical tool shows insufficient specificity on target proteins. This chapter describes a brief overview and practical application of the auxin chemical tools.