Science translational medicine, 2019-01, Vol.11 (476)
2019
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- Autor(en) / Beteiligte
- Titel
- Hypertrophic cardiomyopathy mutations in MYBPC3 dysregulate myosin
- Ist Teil von
- Science translational medicine, 2019-01, Vol.11 (476)
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The mechanisms by which truncating mutations in (encoding cardiac myosin-binding protein C; cMyBPC) or myosin missense mutations cause hypercontractility and poor relaxation in hypertrophic cardiomyopathy (HCM) are incompletely understood. Using genetic and biochemical approaches, we explored how depletion of cMyBPC altered sarcomere function. We demonstrated that stepwise loss of cMyBPC resulted in reciprocal augmentation of myosin contractility. Direct attenuation of myosin function, via a damaging missense variant (F764L) that causes dilated cardiomyopathy (DCM), normalized the increased contractility from cMyBPC depletion. Depletion of cMyBPC also altered dynamic myosin conformations during relaxation, enhancing the myosin state that enables ATP hydrolysis and thin filament interactions while reducing the super relaxed conformation associated with energy conservation. MYK-461, a pharmacologic inhibitor of myosin ATPase, rescued relaxation deficits and restored normal contractility in mouse and human cardiomyocytes with mutations. These data define dosage-dependent effects of cMyBPC on myosin that occur across the cardiac cycle as the pathophysiologic mechanisms by which truncations cause HCM. Therapeutic strategies to attenuate cMyBPC activity may rescue depressed cardiac contractility in patients with DCM, whereas inhibiting myosin by MYK-461 should benefit the substantial proportion of patients with HCM with mutations.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1946-6234eISSN: 1946-6242DOI: 10.1126/scitranslmed.aat1199Titel-ID: cdi_pubmed_primary_30674652
- Format
- –
- Schlagworte
- Adenosine Triphosphate - analogs & derivatives, Adenosine Triphosphate - metabolism, Animals, Cardiomyopathy, Hypertrophic - genetics, Cardiomyopathy, Hypertrophic - physiopathology, Carrier Proteins - genetics, Disease Models, Animal, Haploinsufficiency, Humans, Mice, Mutation - genetics, Myocardial Contraction, Myocardium - metabolism, Myocardium - pathology, Myocytes, Cardiac - metabolism, Myosins - metabolism, ortho-Aminobenzoates - metabolism, Phenotype