Methods in molecular biology (Clifton, N.J.), 2019, Vol.1911, p.105
2019
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Details
- Autor(en) / Beteiligte
- Titel
- Production and Purification of Cell Culture Hepatitis C Virus
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2019, Vol.1911, p.105
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Hepatitis C virus (HCV) is a peculiar member of the Flaviviridae family, with features in between an enveloped virus and a human lipoprotein and, consequently, unusual biophysical properties that made its production and purification rather challenging.Here we describe methods to generate HCV stocks in cell culture by electroporating in vitro transcribed viral RNA into permissive cell lines as well as downstream concentration and purification strategies.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-8976-8_7Titel-ID: cdi_pubmed_primary_30593621
- Format
- –
- Schlagworte
- Cell Culture Techniques - instrumentation, Cell Culture Techniques - methods, Cell Line, Electroporation - methods, Equipment Design, Gene Expression Regulation, Viral, Genome, Viral, Hepacivirus - genetics, Hepacivirus - isolation & purification, Hepacivirus - physiology, Hepatitis C - virology, Humans, RNA, Viral - genetics, Transcription, Genetic, Transfection - methods, Ultrafiltration - instrumentation, Ultrafiltration - methods, Virus Replication