Details

Autor(en) / Beteiligte

• Marvin, Jonathan S
• Scholl, Benjamin
• Wilson, Daniel E
• Podgorski, Kaspar
• Kazemipour, Abbas
• Müller, Johannes Alexander
• Schoch, Susanne
• Quiroz, Francisco José Urra
• Rebola, Nelson
• Bao, Huan
• Little, Justin P
• Tkachuk, Ariana N
• Cai, Edward
• Hantman, Adam W
• Wang, Samuel S-H
• DePiero, Victor J
• Borghuis, Bart G
• Chapman, Edwin R
• Dietrich, Dirk
• DiGregorio, David A
• Fitzpatrick, David
• Looger, Loren L

Titel

Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Ist Teil von

• Nature methods, 2018-11, Vol.15 (11), p.936-939

Ort / Verlag

United States

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.