Details

Autor(en) / Beteiligte

• Lai, Binbin
• Tang, Qingsong
• Jin, Wenfei
• Hu, Gangqing
• Wangsa, Darawalee
• Cui, Kairong
• Stanton, Benjamin Z
• Ren, Gang
• Ding, Yi
• Zhao, Ming
• Liu, Shuai
• Song, Jiuzhou
• Ried, Thomas
• Zhao, Keji

Titel

Trac-looping measures genome structure and chromatin accessibility

Ist Teil von

• Nature methods, 2018-09, Vol.15 (9), p.741

Ort / Verlag

United States

Erscheinungsjahr

2018

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Long-range chromatin interactions play critical roles in genome organization and regulation of transcription. We now report transposase-mediated analysis of chromatin looping (Trac-looping) for simultaneous detection of multiscale genome-wide chromatin interactions among regulatory elements and chromatin accessibility. With this technique, a bivalent oligonucleotide linker is inserted between two interacting regions such that the chromatin interactions are captured without prior chromatin fragmentation and proximity-based ligation. Application of Trac-looping to human CD4 T cells revealed substantial reorganization of enhancer-promoter interactions associated with changes in gene expression after T cell receptor stimulation.