Annual review of cell and developmental biology, 2018-10, Vol.34, p.163
2018
Details
- Autor(en) / Beteiligte
- Titel
- Sortase A: A Model for Transpeptidation and Its Biological Applications
- Ist Teil von
- Annual review of cell and developmental biology, 2018-10, Vol.34, p.163
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Molecular biologists and chemists alike have long sought to modify proteins with substituents that cannot be installed by standard or even advanced genetic approaches. We here describe the use of transpeptidases to achieve these goals. Living systems encode a variety of transpeptidases and peptide ligases that allow for the enzyme-catalyzed formation of peptide bonds, and protein engineers have used directed evolution to enhance these enzymes for biological applications. We focus primarily on the transpeptidase sortase A, which has become popular over the past few years for its ability to perform a remarkably wide variety of protein modifications, both in vitro and in living cells.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1530-8995DOI: 10.1146/annurev-cellbio-100617-062527Titel-ID: cdi_pubmed_primary_30110557
- Format
- –
- Schlagworte
- Amino Acid Sequence - genetics, Aminoacyltransferases - chemistry, Aminoacyltransferases - genetics, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Catalysis, Cysteine Endopeptidases - chemistry, Cysteine Endopeptidases - genetics, Humans, Peptides - chemistry, Peptides - genetics, Peptidyl Transferases - chemistry, Peptidyl Transferases - genetics, Protein Engineering, Substrate Specificity