Details

Autor(en) / Beteiligte

• Chen, Fuzuo
• Song, Huifeng
• Guo, Ximin
• Yue, Xiaotong
• Liu, Lingying
• Zhou, Yong
• Liu, Donghua
• Qian, Zhiyong
• Wang, Tongmin

Titel

Effects of PRX-2 gene on the phenotype changes of epidermal stem cells differentiating into sweat gland cells

Ist Teil von

• Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery, 2017-01, Vol.33 (1), p.37

Ort / Verlag

China

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effects of PRX-2 gene on phenotype changes in epidermal stem cells differentiating into sweat gland cells. Epidermal stem cells and sweat gland cells separated and cultured from healthy foreskin and adult full-thick skin respectively, were identified by immunofluorescence staining. Lentiviral vector-mediated overexpression and knockdown of PRX-2 gene in epidermal stem cells were performed respectively,with empty vector-mediated epidermal stem cells as a control group. Overexpression blank control and know down group's PRX-2 expressions in gene and protein levels were detected using RT-PCR and Western blot technology. The ESCs of each group were co-cultured with sweat gland cells through transwell plate, and the expressions of CEA and β1 integrin in epidermal stem cells were determined by flow cytometry before and after co-culturing. Epidermal stem cells and sweat gland cells were in line with their respective specific antigens .Before co-cultured, epidermal stem cells highly expressed β1 in