Details

Autor(en) / Beteiligte

• Drapek, Colleen
• Sparks, Erin E
• Marhavy, Peter
• Taylor, Isaiah
• Andersen, Tonni G
• Hennacy, Jessica H
• Geldner, Niko
• Benfey, Philip N

Titel

Minimum requirements for changing and maintaining endodermis cell identity in the Arabidopsis root

Ist Teil von

• Nature plants, 2018-08, Vol.4 (8), p.586-595

Ort / Verlag

England

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Changes in gene regulation during differentiation are governed by networks of transcription factors. The Arabidopsis root endodermis is a tractable model to address how transcription factors contribute to differentiation. We used a bottom-up approach to understand the extent to which transcription factors that are required for endodermis differentiation can confer endodermis identity to a non-native cell type. Our results show that the transcription factors SHORTROOT and MYB36 alone have limited ability to induce ectopic endodermal features in the absence of additional cues. The stele-derived signalling peptide CIF2 stabilizes SHORTROOT-induced endodermis identity acquisition. The outcome is a partially impermeable barrier deposited in the subepidermal cell layer, which has a transcriptional signature similar to the endodermis. These results demonstrate that other root cell types can be forced to differentiate into the endodermis and highlight a previously unappreciated role for receptor kinase signalling in maintaining endodermis identity.