Details

Autor(en) / Beteiligte

• Luo, Y X
• Guo, J B
• Yin, F R
• Huo, X X
• Zheng, L B
• Zhang, H
• Zhang, X L

Titel

The effects of macrophages with high expression of TL1A on activation and proliferation of hepatic stellate cells in vitro

Ist Teil von

• Zhonghua gan zang bing za zhi, 2018-05, Vol.26 (5), p.347

Ort / Verlag

China

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• To explore the effects of macrophages with high expression of TL1A on the activation and proliferation of HSCs in vitro. The Bone marrow-derived macrophages (BMMs) and peritoneal macrophages (PMs) from wild type (WT) and myeloid-overexpressed TL1A transgenic mice were isolated, differentiated and activated. HSCs were harvested from activated macrophages culture supernatant (CM). HSCs were detected by immunofluorescence and real-time Q-PCR. And the proliferation was detected by CCK-8 and BrdU assay kit. The levels of IL-1β and PDGF-BB in macrophage culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). BMMs-derived CM-intervention HSCs were used to detect the expression of α-smooth muscle actin (α-SMA) on the 2nd, 4th and 6th day respectively by immunofluorescence method. There was no significant difference between the two groups on the 2 nd and the 6th day, > 0.05; On day 4, the CM/Tg group was significantly higher than that of CM/WT group, < 0.01; the results of CMs derived from P