Nature (London), 2018-07, Vol.559 (7714), p.423
2018
Details
- Autor(en) / Beteiligte
- Titel
- PtdIns(4,5)P 2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling
- Ist Teil von
- Nature (London), 2018-07, Vol.559 (7714), p.423
- Ort / Verlag
- England
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- G-protein-coupled receptors (GPCRs) are involved in many physiological processes and are therefore key drug targets . Although detailed structural information is available for GPCRs, the effects of lipids on the receptors, and on downstream coupling of GPCRs to G proteins are largely unknown. Here we use native mass spectrometry to identify endogenous lipids bound to three class A GPCRs. We observed preferential binding of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P ) over related lipids and confirm that the intracellular surface of the receptors contain hotspots for PtdIns(4,5)P binding. Endogenous lipids were also observed bound directly to the trimeric Gα βγ protein complex of the adenosine A receptor (A R) in the gas phase. Using engineered Gα subunits (mini-Gα mini-Gα and mini-Gα ) , we demonstrate that the complex of mini-Gα with the β adrenergic receptor (β AR) is stabilized by the binding of two PtdIns(4,5)P molecules. By contrast, PtdIns(4,5)P does not stabilize coupling between β AR and other Gα subunits (mini-Gα or mini-Gα ) or a high-affinity nanobody. Other endogenous lipids that bind to these receptors have no effect on coupling, highlighting the specificity of PtdIns(4,5)P . Calculations of potential of mean force and increased GTP turnover by the activated neurotensin receptor when coupled to trimeric Gα βγ complex in the presence of PtdIns(4,5)P provide further evidence for a specific effect of PtdIns(4,5)P on coupling. We identify key residues on cognate Gα subunits through which PtdIns(4,5)P forms bridging interactions with basic residues on class A GPCRs. These modulating effects of lipids on receptors suggest consequences for understanding function, G-protein selectivity and drug targeting of class A GPCRs.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1476-4687DOI: 10.1038/s41586-018-0325-6Titel-ID: cdi_pubmed_primary_29995853
- Format
- –
- Schlagworte
- Animals, GTP-Binding Protein alpha Subunits, Gi-Go - metabolism, GTP-Binding Protein alpha Subunits, Gs - metabolism, Heterotrimeric GTP-Binding Proteins - metabolism, Humans, Molecular Dynamics Simulation, Phosphatidylinositol 4,5-Diphosphate - metabolism, Protein Stability, Rats, Receptors, Adrenergic, alpha-2 - chemistry, Receptors, Adrenergic, alpha-2 - genetics, Receptors, Adrenergic, alpha-2 - metabolism, Receptors, Adrenergic, beta-1 - chemistry, Receptors, Adrenergic, beta-1 - genetics, Receptors, Adrenergic, beta-1 - metabolism, Receptors, G-Protein-Coupled - chemistry, Receptors, G-Protein-Coupled - genetics, Receptors, G-Protein-Coupled - metabolism, Receptors, Neurotensin - chemistry, Receptors, Neurotensin - genetics, Receptors, Neurotensin - metabolism, Single-Chain Antibodies - chemistry, Single-Chain Antibodies - metabolism, Substrate Specificity, Turkeys