Details

Autor(en) / Beteiligte

• Zallet, J
• Olaru, I D
• Witt, A-K
• Vock, P
• Kalsdorf, B
• Andres, S
• Hillemann, D
• Kranzer, K

Titel

Evaluation of OMNIgene ® •SPUTUM reagent for mycobacterial culture

Ist Teil von

• The international journal of tuberculosis and lung disease, 2018-08, Vol.22 (8), p.945-949

Ort / Verlag

France

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• National Mycobacterium Reference Laboratory, Borstel, Germany. To evaluate the effectiveness of OMNIgene®•SPUTUM (OM-S) reagent in comparison with a method using N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) with regard to mycobacterial recovery and contamination of broth and solid cultures. Sputum samples from patients with tuberculosis and other respiratory diseases underwent decontamination with NALC-NaOH-based (MycoDDR™) or OM-S reagent. The decontamination procedure was assigned by block randomisation. Samples were inoculated on Löwenstein-Jensen, Stonebrink and MGIT™ (Mycobacterial Growth Indicator Tubes). Mycobacterial recovery from samples spiked with Mycobacterium tuberculosis following decontamination was determined. Eighty-five samples were randomised to NALC-NaOH and 84 to OM-S reagent. Mycobacterial recovery was significantly lower for samples processed with OM-S reagent compared with the NALC-NaOH method across all media types. Culture contamination was lower with NALC-NaOH reagent on solid media (9.4-12.9% vs. 28.6-29.8%). Growth was not observed in MGIT among samples spiked with 10 600-16 800 colony-forming units of M. tuberculosis following decontamination with OM-S reagent. Low mycobacterial recovery, especially in MGIT, observed in the present study suggests that OM-S reagent might not be compatible with the MGIT system. More extensive field evaluations of the OM-S reagent are warranted to demonstrate a significant benefit over currently used methods.