Details

Autor(en) / Beteiligte

• Gao, X-L
• Cao, M-G
• Ai, G-G
• Hu, Y-B

Titel

Mir-98 reduces the expression of HMGA2 and promotes osteogenic differentiation of mesenchymal stem cells

Ist Teil von

• European review for medical and pharmacological sciences, 2018-06, Vol.22 (11), p.3311

Ort / Verlag

Italy

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• To study effects of microRNA-98 (miR-98) on osteogenic differentiation of mesenchymal stem cells (MSCs). We predicted target gene of miR-98 with software test, and detected expression changes of miR-98, as well as its target gene HMGA2, in the process of osteogenic differentiation of mesenchymal stem cells. After transfection of miR-98 mimic and HMGA2 siRNA, we induced osteogenic differentiation and detected expression changes of osteogenic differentiation markers (RUNX2, ALP, OCN, and BSP). MiR-98 combined directly with target gene HMGA2 and inhibited its expression. During the process of osteogenic differentiation, expression of miR-98 was up-regulated, while HMGA2 expression was down-regulated. In addition, the expression of osteogenesis maker genes increased in cells being transfected with miR-98 mimics and HMGA2 siRNA. MiR-98 can promote osteogenic differentiation of mesenchymal stem cells by targeting gene HMGA2.