Methods in molecular biology (Clifton, N.J.), 2018-01, Vol.1782, p.121
2018
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Details
- Autor(en) / Beteiligte
- Titel
- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2018-01, Vol.1782, p.121
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-7831-1_7Titel-ID: cdi_pubmed_primary_29850997
- Format
- –
- Schlagworte
- Animals, Cell Culture Techniques - instrumentation, Cell Culture Techniques - methods, Cells, Cultured, Cerebellum - cytology, Fluorescence, Fluorescent Dyes - chemistry, Membrane Potential, Mitochondrial, Microscopy, Confocal - instrumentation, Microscopy, Confocal - methods, Microscopy, Fluorescence - instrumentation, Microscopy, Fluorescence - methods, Mitochondria - metabolism, Neurons, Rats, Rhodamine 123 - chemistry, Rhodamines - chemistry, Single-Cell Analysis - instrumentation, Single-Cell Analysis - methods