Methods in molecular biology (Clifton, N.J.), 2018-01, Vol.1792, p.1
2018
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Details
- Autor(en) / Beteiligte
- Titel
- ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2018-01, Vol.1792, p.1
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A quantitative allele-specific polymerase chain reaction in combination with an extreme limiting dilution approach (ELDA qASO-PCR) enables the detection of tumor cells in patients with multiple myeloma (MM) in bone marrow (BM) samples and in peripheral blood (PB) with a sensitivity of <10 . The two-step procedure of patient-specific tumor cell identification via the immunoglobulin heavy chain (IgH) and kappa/lambda light chain (k/λ LC) locus, followed by tumor cells quantification by ELDA qASO-PCR allows for the application of this method to the majority of MM patients, including those with Bence Jones proteinuria.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-7865-6_1Titel-ID: cdi_pubmed_primary_29797248
- Format
- –
- Schlagworte
- Alleles, Base Sequence, Gene Rearrangement, B-Lymphocyte, Heavy Chain, Humans, Immunoglobulin Heavy Chains - genetics, Neoplasm, Residual - diagnosis, Neoplasm, Residual - genetics, Neoplasms - diagnosis, Neoplasms - genetics, Neoplastic Cells, Circulating - metabolism, Neoplastic Cells, Circulating - pathology, Polymerase Chain Reaction - methods, Sensitivity and Specificity