Details

Autor(en) / Beteiligte

• Gladstein, Scott
• Stawarz, Andrew
• Almassalha, Luay M
• Cherkezyan, Lusik
• Chandler, John E
• Zhou, Xiang
• Subramanian, Hariharan
• Backman, Vadim

Titel

Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2018, Vol.1745, p.337

Ort / Verlag

United States

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Despite extensive research in the area, current understanding of the structural organization of higher-order chromatin topology (between 20 and 200 nm) is limited due to a lack of proper imaging techniques at these length scales. The organization of chromatin at these scales defines the physical context (nanoenvironment) in which many important biological processes occur. Improving our understanding of the nanoenvironment is crucial because it has been shown to play a critical functional role in the regulation of chemical reactions. Recent progress in partial wave spectroscopic (PWS) microscopy enables real-time measurement of higher-order chromatin organization within label-free live cells. Specifically, PWS quantifies the nanoscale variations in mass density (heterogeneity) within the cell. These advancements have made it possible to study the functional role of chromatin topology, such as its regulation of the global transcriptional state of the cell and its role in the development of cancer. In this chapter, the importance of studying chromatin topology is explained, the theory and instrumentation of PWS are described, the measurements and analysis processes for PWS are laid out in detail, and common issues, troubleshooting steps, and validation techniques are provided.