Details

Autor(en) / Beteiligte

• Bao, Xichen
• Guo, Xiangpeng
• Yin, Menghui
• Tariq, Muqddas
• Lai, Yiwei
• Kanwal, Shahzina
• Zhou, Jiajian
• Li, Na
• Lv, Yuan
• Pulido-Quetglas, Carlos
• Wang, Xiwei
• Ji, Lu
• Khan, Muhammad J
• Zhu, Xihua
• Luo, Zhiwei
• Shao, Changwei
• Lim, Do-Hwan
• Liu, Xiao
• Li, Nan
• Wang, Wei
• He, Minghui
• Liu, Yu-Lin
• Ward, Carl
• Wang, Tong
• Zhang, Gong
• Wang, Dongye
• Yang, Jianhua
• Chen, Yiwen
• Zhang, Chaolin
• Jauch, Ralf
• Yang, Yun-Gui
• Wang, Yangming
• Qin, Baoming
• Anko, Minna-Liisa
• Hutchins, Andrew P
• Sun, Hao
• Wang, Huating
• Fu, Xiang-Dong
• Zhang, Biliang
• Esteban, Miguel A

Titel

Capturing the interactome of newly transcribed RNA

Ist Teil von

• Nature methods, 2018-03, Vol.15 (3), p.213-220

Ort / Verlag

United States

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.