Details

Autor(en) / Beteiligte

• Bulk, Etmar
• Kramko, Nadzeya
• Liashkovich, Ivan
• Glaser, Felix
• Schillers, Hermann
• Schnittler, Hans-Joachim
• Oberleithner, Hans
• Schwab, Albrecht

Titel

K Ca 3.1 channel inhibition leads to an ICAM-1 dependent increase of cell-cell adhesion between A549 lung cancer and HMEC-1 endothelial cells

Ist Teil von

• Oncotarget, 2017-12, Vol.8 (68), p.112268

Ort / Verlag

United States

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Early metastasis leads to poor prognosis of lung cancer patients, whose 5-year survival rate is only 15%. We could recently show that the Ca sensitive K channel K 3.1 promotes aggressive behavior of non-small cell lung cancer (NSCLC) cells and that it can serve as a prognostic marker in NSCLC. Since NSCLC patients die of metastases, we investigated whether K 3.1 channels contribute to poor patient prognosis by regulating distinct steps of the metastatic cascade. We investigated the extravasation of NSCLC cells and focused on their adhesion to endothelial cells and on transendothelial migration. We quantified the adhesion forces between NSCLC cells and endothelial cells by applying single cell force spectroscopy, and we monitored transendothelial migration using live-cell imaging. Inhibition of K 3.1 channels with senicapoc or K 3.1 silencing increases the adhesion force of A549 lung cancer cells to human microvascular endothelial cells (HMEC-1). Western blotting, immunofluorescence staining and biotinylation assays indicate that the elevated adhesion force is due to increased expression of ICAM-1 in both cell lines when K 3.1 channels are downregulated. Consistent with this interpretation, an anti-ICAM-1 blocking antibody abolishes the K 3.1-dependent increase in adhesion. Senicapoc inhibits transendothelial migration of A549 cells by 50%. Selectively silencing K 3.1 channels in either NSCLC or endothelial cells reveals that transendothelial migration depends predominantly on endothelial K 3.1 channels. In conclusion, our findings disclose a novel function of K 3.1 channels in cancer. K 3.1 channels regulate ICAM-1 dependent cell-cell adhesion between endothelial and cancer cells that affects the transmigration step of the metastatic cascade.