Details

Autor(en) / Beteiligte

• de Souza Ferreira, Ederlan
• Capraro, Jessica
• Sessa, Fabio
• Magni, Chiara
• Demonte, Aureluce
• Consonni, Alessandro
• Augusto Neves, Valdir
• Maffud Cilli, Eduardo
• Duranti, Marcello
• Scarafoni, Alessio

Titel

New molecular features of cowpea bean (Vigna unguiculata, l. Walp) β-vignin

Ist Teil von

• Bioscience, biotechnology, and biochemistry, 2018-02, Vol.82 (2), p.285-291

Ort / Verlag

England: Taylor & Francis

Erscheinungsjahr

2018

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Cowpea seed β-vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of β-vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins. Using a shorted protocol, -vignin with a high degree of purity was obtain. A glycolylated gene product, never identified before, and its metal binding capacity were identified.