Methods in molecular biology (Clifton, N.J.), 2018, Vol.1722, p.151
2018
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- Autor(en) / Beteiligte
- Titel
- Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4
- Ist Teil von
- Methods in molecular biology (Clifton, N.J.), 2018, Vol.1722, p.151
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- G protein-coupled receptors (GPCRs) are cell surface receptors that relay extracellular signals to the inside of the cells. C-X-C chemokine receptor 4 (CXCR4) is a GPCR that undergoes receptor internalization and recycling upon stimulation with its cognate ligand, C-X-C chemokine 12 (CXCL12). Using this receptor/ligand pair we describe the use of two techniques, enzyme-linked immunosorbent assay (ELISA) and flow cytometry, widely used to quantify GPCR internalization from the plasma membrane and its return to the cell surface by recycling.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1940-6029DOI: 10.1007/978-1-4939-7553-2_10Titel-ID: cdi_pubmed_primary_29264804
- Format
- –
- Schlagworte
- Absorption, Physicochemical, Antibodies, Monoclonal - chemistry, Cell Membrane - chemistry, Chemokine CXCL12 - chemistry, Chemokine CXCL12 - metabolism, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Dyes - chemistry, HEK293 Cells, HeLa Cells, Humans, Ligands, Receptors, CXCR4 - analysis, Receptors, CXCR4 - biosynthesis, Receptors, CXCR4 - chemistry