Details

Autor(en) / Beteiligte

• Yang, Yong
• Guo, Xin
• Yu, Zhi-Cheng
• Ma, Jia-Qing
• Liu, Xing-Ling
• Li, Li-Sha
• Xiao, Gao-Peng
• Liu, Xiao-Meng
• Li, Xin-Nan
• Shen, Jin-Song
• Li, Yan-Hua
• Liu, Rui

Titel

Relationship between cytoplasmic phospholipase A2 and nuclear factor κB in one lung ventilation-induced lung injury in rabbits

Ist Teil von

• Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2017-10, Vol.37 (10), p.1345

Ort / Verlag

China

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• To elucidate the mechanisms of up regulated expression of cytoplasmic phospholipase A2 (CPLA2) induced by one lung ventilation (OLV) by investigating the interactions between nuclear factor kappaB (NF-κB) and C-PLA2. Forty-eight healthy Japanese white rabbits were randomized into control group, solvent treatment group (group S), NF-κB inhibitor (PDTC)/solvent treatment group ( group PS), C-PLA2 inhibitor (AACOCF3)/solvent treatment group (group AS), OLV group (group O), solvent treatment plus OLV group (SO group), NFκB inhibitor (PDTC)/solvent treatment plus OLV group (group PSO) and CPLA2 inhibitor (AACOCF3)/solvent treatment plus OLV group (group ASO). ELISA was used to detect arachidonic acid (AA) content in the lung tissues, and NFκB and CPLA2 expressions were detected by Western blotting and quantitative PCR. Lung injuries were assessed based on the lung histological score, and the polymorphonuclear leukocyte count in the bronchial alveolar lavage fluid, myeloperoxidase (MPO) content in the lung tissues,