Details

Autor(en) / Beteiligte

• Gan, Zhen
• Wu, Hao
• Wu, Hao-Han
• Zhou, Mei-Jun
• Yan, Fei
• Liu, Hong-Mei

Titel

Efficacy of internalized RGD-modified echogenic liposomes in diagnosis and treatment in a mouse model of rheumatoid arthritis

Ist Teil von

• Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2017-10, Vol.37 (10), p.1283

Ort / Verlag

China

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• To prepare internalized RGD (iRGD) modified echogenic liposomes containing methotrexate (MTX) and indocyanine green (ICG) (iRGD MTX ICG ELIP) and evaluate its targeting efficiency and inhibitory effect combined with ultrasound on synovial cells. iRGD MTX ICG ELIP was prepared by the thin film rehydration and freeze-lyophilization method and its general characteristics and acoustic responsiveness were assessed. The targeting effect of the prepared liposome was observed by assessing its cell uptake in vitro. In a mouse model of rheumatiod arthritis, the targeting effect of the prepared liposome was determined by detecting the fluorescence intensity of the drug in arthrosis. The inhibitory effect of iRGD MTX ICG ELIP combined with ultrasound on synovial MH7A cells in vitro were investigated using CCK8 test. The average diameter and zeta potential of iRGD MTX ICG ELIP was 134.4∓17.61 nm and 10.07∓4.28 mV, and the entrapment efficiency of MTX and ICG was (62.56∓0.77)% and (95.13∓0.82)%, respectively. With ultrasou