Journal of Alzheimer's disease, 2017-01, Vol.60 (3), p.1143-1160
2017
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- Autor(en) / Beteiligte
- Titel
- Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain
- Ist Teil von
- Journal of Alzheimer's disease, 2017-01, Vol.60 (3), p.1143-1160
- Ort / Verlag
- Netherlands
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by the presence of intracellular neurofibrillary tangles (NFTs) containing hyperphosphorylated tau, and the extracellular deposition of amyloid plaques (APs) with misfolded amyloid-β (Aβ) peptide. Glia maturation factor (GMF), a highly conserved pro-inflammatory protein, isolated and cloned in our laboratory, has been shown to activate glial cells leading to neuroinflammation and neurodegeneration in AD. We hypothesized that inflammatory reactions promoted by NLRP3-Caspase-1inflammasome pathway trigger dysfunction in autophagy and accumulation of Aβ which is amplified and regulated by GMF in AD. In this study, using immunohistochemical techniques we analyzed components of the NLRP3 inflammasome and autophagy- lysosomal markers in relation to Aβ, p-tau and GMF in human postmortem AD and age-matched non-AD brains. Tissue sections were prepared from the temporal cortex of human postmortem brains. Here, we demonstrate an increased expression of the inflammasome components NLRP3 and Caspase-1 and the products of inflammasome activation IL-1β and IL-18 along with GMF in the temporal cortex of AD brains. These inflammasome components and the pro-inflammatory cytokines co-localized with GMF in the vicinity and periphery of the APs and NFTs. Moreover, using double immunofluorescence staining, AD brain displayed an increase in the autophagy SQSTM1/p62 and LC3 positive vesicles and the lysosomal marker LAMP1 that also co-localized with GMF, Aβ and hyperphosphorylated p-tau. Our results indicate that in AD, the neuroinflammation promoted by the NLRP3 inflammasome may be amplified and regulated by GMF, which further impairs clearance of protein aggregates mediated by the auto-phagosomal pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1387-2877eISSN: 1875-8908DOI: 10.3233/JAD-170634Titel-ID: cdi_pubmed_primary_28984607
- Format
- –
- Schlagworte
- Alzheimer Disease - metabolism, Alzheimer Disease - pathology, Amyloid beta-Peptides - metabolism, Autophagosomes - metabolism, Calcium-Binding Proteins, Caspase 1 - metabolism, DNA-Binding Proteins - metabolism, Fluorescent Antibody Technique, Glia Maturation Factor - metabolism, Glial Fibrillary Acidic Protein - metabolism, Humans, Inflammasomes - metabolism, Interleukin-18 - metabolism, Interleukin-1beta - metabolism, Lysosomal Membrane Proteins - metabolism, Microfilament Proteins, Microtubule-Associated Proteins - metabolism, NLR Family, Pyrin Domain-Containing 3 Protein - metabolism, Phosphorylation, Plaque, Amyloid - metabolism, Plaque, Amyloid - pathology, RNA-Binding Proteins - metabolism, Sequestosome-1 Protein - metabolism, tau Proteins - metabolism, Temporal Lobe - metabolism, Temporal Lobe - pathology