Zhongguo zhongyao zazhi, 2017-01, Vol.42 (2), p.220
2017
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Cloning and protein expression analysis of geranyl diphosphate synthase genes in Tripterygium wilfordii
- Ist Teil von
- Zhongguo zhongyao zazhi, 2017-01, Vol.42 (2), p.220
- Ort / Verlag
- China
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Based on the transcriptome data, the study cloned full-length cDNA of TwGPPS1 and TwGPPS2 genes from Tripterygium wilfordii suspension cells and then analyzed the bioinformation of the sequence and protein expression. The cloned TwGPPS1 has a 1 278 bp open reading frame (ORF) encoding a polypeptide of 425 amino acids. The deduced isoelectric point (pI) was 6.68, a calculated molecular weight was about 47.189 kDa. The full-length cDNA of the TwGPPS2 contains a 1 269 bp open reading frame (ORF) encoding a polypeptide of 422 amino acids. The deduced isoelectric point (pI) was 6.71, a calculated molecular weight was about 46.774 kDa.The entire reading frame of TwGPPS1,2 was cloned into the pET-32a(+) vector and expressed in E. coli BL21 (DE3) cells to obtain the TwGPPS protein, which laid a basis for further study on the regulation of terpenoid secondary metabolism and biological synthesis.
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1001-5302DOI: 10.19540/j.cnki.cjcmm.20161222.042Titel-ID: cdi_pubmed_primary_28948723