Details

Autor(en) / Beteiligte

• Hsu, Phillip J
• Zhu, Yunfei
• Ma, Honghui
• Guo, Yueshuai
• Shi, Xiaodan
• Liu, Yuanyuan
• Qi, Meijie
• Lu, Zhike
• Shi, Hailing
• Wang, Jianying
• Cheng, Yiwei
• Luo, Guanzheng
• Dai, Qing
• Liu, Mingxi
• Guo, Xuejiang
• Sha, Jiahao
• Shen, Bin
• He, Chuan

Titel

Ythdc2 is an N^6-methyladenosine binding protein that regulates mammalian spermatogenesis

Ist Teil von

• Cell research, 2017-09, Vol.27 (9), p.1115-1127

Ort / Verlag

England

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• N^6-methyladenosine （m^6A） is the most common internal modification in eukaryotic mRNA. It is dynamically in- stalled and removed, and acts as a new layer of mRNA metabolism, regulating biological processes including stem cell pluripotency, cell differentiation, and energy homeostasis, m^6A is recognized by selective binding proteins; YTHDF1 and YTHDF3 work in concert to affect the translation of m^6A-containing mRNAs, YTHDF2 expedites mRNA decay, and YTHDC1 affects the nuclear processing of its targets. The biological function of YTHDC2, the final member of the YTH protein family, remains unknown. We report that YTHDC2 selectively binds m6A at its consensus motif. YTHDC2 enhances the translation efficiency of its targets and also decreases their mRNA abundance. Ythdc2 knock- out mice are infertile; males have significantly smaller testes and females have significantly smaller ovaries compared to those oflittermates. The germ cells of Ythdc2 knockout mice do not develop past the zygotene stage and according- ly, Ythdc2 is upregulated in the testes as meiosis begins. Thus, YTHDC2 is an m6A-binding protein that plays critical roles during svermatogenesis.