Details

Autor(en) / Beteiligte

• Wang, Wei-Li
• Wang, Le
• Hu, Tian-Yuan
• Li, Cong
• Yuan, Wei-Ping

Titel

Effect of PDK1 on Neutrophil Differentiation

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2017-06, Vol.25 (3), p.932

Ort / Verlag

China

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil. The PDK1 expression level of Vav-Cre;PDK1 mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU-C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis. The RNA expression level of bone marrow cells of Vav-Cre;PDK1 mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDK1 mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre;PDK1 group compared with control groups. PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.