Details

Autor(en) / Beteiligte

• Hoppmann, Christian
• Wong, Allison
• Yang, Bing
• Li, Shuwei
• Hunter, Tony
• Shokat, Kevan M
• Wang, Lei

Titel

Site-specific incorporation of phosphotyrosine using an expanded genetic code

Ist Teil von

• Nature chemical biology, 2017-08, Vol.13 (8), p.842-844

Ort / Verlag

United States

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.