Details

Autor(en) / Beteiligte

• Li, Ping
• Zhang, Min
• Ma, Jian-Qiang
• Sun, Qi
• Liu, Guang-Xiang
• Zhao, Xiao-Zhi
• Feng, Wen-Huan
• Shen, Shan-Mei
• Guo, Hong-Qian
• Zhu, Da-Long

Titel

Expression and Histopathological Significance of Disabled-2 in Aldosterone-Producing Adenoma

Ist Teil von

• Hormone and metabolic research, 2017-07, Vol.49 (7), p.520

Ort / Verlag

Germany

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• The current pathological diagnosis of aldosterone-producing adenoma (APA) is challenging because no histological markers of aldosterone production are available in routine practice. A previous study demonstrated that Disabled-2 (DAB2) is a specific marker of the ( ) in rodents. The aim of the present study was to investigate the significance of immunohistochemical staining to detect DAB2 in the adrenal tissue of patients with APA. We investigated the expression of DAB2 in 36 adrenal glands with APA, 23 adrenal glands with cortisol-producing adenoma (CPA), and 33 adrenal glands with non-functioning adenoma (NFA). Immunohistochemical staining was performed using anti-DAB2 antibodies on paraffin-embedded sections. We analysed the expression of DAB2 semi-quantitatively by scoring staining intensity, and assessed the correlation of this information with the clinical findings. mRNA expression in adenoma tissues was evaluated by RT-PCR. DAB2 was highly expressed in the in normal human adrenal glands. DAB2 expression was heterogeneous in APA, with spotted, strong staining noted in most samples (25 of 36 APA). CPA and NFA also exhibited extensive low or moderate DAB2 expression. mRNA was significantly increased and positively correlated with in APA (p<0.05). In APA, the DAB2 score adjusted for tumour volume was positively correlated with plasma aldosterone (p<0.05). Patients with low or moderate DAB2 staining more frequently exhibited high blood pressure and were diagnosed at a younger age compared with patients with high DAB2 staining. The present study clearly demonstrates that DAB2 is a specific marker of the in normal human adrenal glands but that DAB2 immunostaining is not sufficiently powerful for histopathological diagnosis of APA. DAB2 might be involved in excessive aldosterone biosynthesis and correlate with specific clinical characteristics of APA patients.