Details

Autor(en) / Beteiligte

• Désogère, Pauline
• Tapias, Luis F
• Hariri, Lida P
• Rotile, Nicholas J
• Rietz, Tyson A
• Probst, Clemens K
• Blasi, Francesco
• Day, Helen
• Mino-Kenudson, Mari
• Weinreb, Paul
• Violette, Shelia M
• Fuchs, Bryan C
• Tager, Andrew M
• Lanuti, Michael
• Caravan, Peter

Titel

Type I collagen-targeted PET probe for pulmonary fibrosis detection and staging in preclinical models

Ist Teil von

• Science translational medicine, 2017-04, Vol.9 (384)

Ort / Verlag

United States

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Pulmonary fibrosis is scarring of the lungs that can arise from radiation injury, drug toxicity, environmental or genetic causes, and for unknown reasons [idiopathic pulmonary fibrosis (IPF)]. Overexpression of collagen is a hallmark of organ fibrosis. We describe a peptide-based positron emission tomography (PET) probe ( Ga-CBP8) that targets collagen type I. We evaluated Ga-CBP8 in vivo in the bleomycin-induced mouse model of pulmonary fibrosis. Ga-CBP8 showed high specificity for pulmonary fibrosis and high target/background ratios in diseased animals. The lung PET signal and lung Ga-CBP8 uptake (quantified ex vivo) correlated linearly ( = 0.80) with the amount of lung collagen in mice with fibrosis. We further demonstrated that the Ga-CBP8 probe could be used to monitor response to treatment in a second mouse model of pulmonary fibrosis associated with vascular leak. Ex vivo analysis of lung tissue from patients with IPF supported the animal findings. These studies indicate that Ga-CBP8 is a promising candidate for noninvasive imaging of human pulmonary fibrosis.