Details

Autor(en) / Beteiligte

• Unger, Ann-Katrin
• Geimer, Stefan
• Harner, Max
• Neupert, Walter
• Westermann, Benedikt

Titel

Analysis of Yeast Mitochondria by Electron Microscopy

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2017-01, Vol.1567, p.293

Ort / Verlag

United States

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.