Details

Autor(en) / Beteiligte

• Shi, Zhengpeng
• Hu, Yumin
• Chen, Hanqing
• Fu, Fengqing
• Zhang, Xueguang

Titel

Identification and characterization of monoclonal antibody Y4F11 against human B7-H3

Ist Teil von

• Xi bao yu fen zi mian yi xue za zhi, 2016-10, Vol.32 (10), p.1402

Ort / Verlag

China

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective To generate mouse anti-human monoclonal antibodies against the molecules expressed on immune cells from malignant pleural effusions, and identify the antigen recognized by the monoclonal antibody named Y4F11. Methods The cells separated from pleural effusions of lung cancer patients were used to immunize BALB/c mice. Hybridoma technology was used for cell fusion and screening; the monoclonal antibody named Y4F11 was chosen as the object for the following experiment. Flow cytometry was performed to analyze the molecules expressed on naive and activated T, B, NK cells and monocytes; Co-immunoprecipitation was conducted to get the antigen pulled down by Y4F11. Mass spectrometry sequencing and BLASTP were used to identify the antigen molecules. Western blotting, dot-blotting and flow cytometry were utilized to identify Y4F11. Results The study obtained 78 hybridoma clones secreting antibodies stably which could recognize surface molecules on cells from pleural effusions. The hybridoma named Y4F11 was ch